Introduction: What is Gene Ontology (GO)?
It is a bioinformatics initiative that aims to provide a comprehensive, up-to-date knowledge about the functions of genes across all species. GO covers three domains:
(1) Cellular component: The location of the gene in the cell where the gene is expressed and functions.
(2) Molecular function: The product of the gene activity at a molecular level, such as "catalysis" or "transport".
(3) Biological process: The larger processes of a gene product that involve multiple molecular activities, such as DNA repair. [1]
(1) Cellular component: The location of the gene in the cell where the gene is expressed and functions.
(2) Molecular function: The product of the gene activity at a molecular level, such as "catalysis" or "transport".
(3) Biological process: The larger processes of a gene product that involve multiple molecular activities, such as DNA repair. [1]
Results: What are the GO terms of HTRA1?
1. Cellular component:
The human serine protease HTRA1 is annotated to be localized to the 1. plasma membrane, 3. cytosol and secreted in the 2. extracellular matrix(ECM). 1. Plasma membrane: also known as cell membrane, is a lipid bilayer that separates the interior of the cell from the exterior(as marked in dark blue line) . 2. ECM: the region outside the plasma membrane. 3. Cytosol: a semi-fluid matrix that embeds various organelles in the cell. [2] |
2. Molecular function:
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3. Biological process
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Conclusions:
HTRA1 gene encodes a member of the trypsin family of serine proteases. Human serine protease HTRA1 is a serine protease with a variety of targets including ECM proteins such as fibronectin. HTRA1 localizes both in the extracellular space and cytoplasm. The GO terms suggest its role in the regulations of various physiological processes such as proteolysis, IGF binding and cell growth.
Reference:
Header image: https://wallpaperscraft.com/download/dna_spiral_dark_lines_figure_38174/1920x1080
[1]About the GO. (2016). Retrieved March 28, 2024, from Gene Ontology Resource website: [2]https://geneontology.org/docs/introduction-to-go[2]Dictionary - The Human Protein Atlas. (2014). Retrieved March 28, 2024, from Proteinatlas.org website: https://www.proteinatlas.org/learn/dictionary/cell
[3]UniProt. (2024). Retrieved March 28, 2024, from Uniprot.org website: https://www.uniprot.org/uniprotkb/Q92743/entry#structure
[4]Sabino, F., Elizabeta Madzharova, & Ulrich. (2020). Cell density-dependent proteolysis by HtrA1 induces translocation of zyxin to the nucleus and increased cell survival. Cell Death and Disease, 11(8). https://doi.org/10.1038/s41419-020-02883-2
[5]Database, G. (2024). HTRA1 Gene - GeneCards | HTRA1 Protein | HTRA1 Antibody. Retrieved March 28, 2024, from Genecards.org website: https://www.genecards.org/cgi-bin/carddisp.pl?gene=HTRA1#function
Header image: https://wallpaperscraft.com/download/dna_spiral_dark_lines_figure_38174/1920x1080
[1]About the GO. (2016). Retrieved March 28, 2024, from Gene Ontology Resource website: [2]https://geneontology.org/docs/introduction-to-go[2]Dictionary - The Human Protein Atlas. (2014). Retrieved March 28, 2024, from Proteinatlas.org website: https://www.proteinatlas.org/learn/dictionary/cell
[3]UniProt. (2024). Retrieved March 28, 2024, from Uniprot.org website: https://www.uniprot.org/uniprotkb/Q92743/entry#structure
[4]Sabino, F., Elizabeta Madzharova, & Ulrich. (2020). Cell density-dependent proteolysis by HtrA1 induces translocation of zyxin to the nucleus and increased cell survival. Cell Death and Disease, 11(8). https://doi.org/10.1038/s41419-020-02883-2
[5]Database, G. (2024). HTRA1 Gene - GeneCards | HTRA1 Protein | HTRA1 Antibody. Retrieved March 28, 2024, from Genecards.org website: https://www.genecards.org/cgi-bin/carddisp.pl?gene=HTRA1#function