Introduction: What is transcriptomics?
Transcriptomics is the study of all RNA molecules in the cell. The suffix "-omics" refers to the large-scale and data-rich study of a specific biological field. According to the central dogma of molecular biology, the genetic information flows in the following order:
DNA → RNA → protein
The transcription process is where the DNA is transcribed into RNA. A transcriptome captures the entire transcripts in the cell.
The transcriptomic data based on RNA-Seq and microarrays methods provides valuable information on gene expression profile in different cell types and organisms. This allows us to investigate into the functions of genes, physiological pathways of human diseases and biological response to different therapeutic treatments.
Transcriptomics is the study of all RNA molecules in the cell. The suffix "-omics" refers to the large-scale and data-rich study of a specific biological field. According to the central dogma of molecular biology, the genetic information flows in the following order:
DNA → RNA → protein
The transcription process is where the DNA is transcribed into RNA. A transcriptome captures the entire transcripts in the cell.
The transcriptomic data based on RNA-Seq and microarrays methods provides valuable information on gene expression profile in different cell types and organisms. This allows us to investigate into the functions of genes, physiological pathways of human diseases and biological response to different therapeutic treatments.
Discussion
The tissue RNA expression of HTRA1 shows an enhanced tissue specificity for ovary and low human brain regional specificity. It is worth noting that HTRA1 RNA and protein expressions are different in female and male tissue, indicating HTRA1 exhibits sexual dimorphism. The discrepancy in RNA and protein expression level of HTRA1 in brain and respiratory system awaits further investigation into the post-translational regulation and other potential regulatory mechanism of HTRA1. Moreover, the tissue expression cluster shows that HTRA1 RNA expression is mainly neuronal transcription. The single cell type specificity shows that HTRA1 is cell type enhanced in Mesothelial cells, Muller glia cells, Fibroblasts and Ovarian stromal cells. Additionally, HTRA1 RNA expression shows no immune cell specificity and low cancer specificity, indicating its fundamental role in various cellular process and limited function in immune response.
Reference:
https://editions.mydigitalpublication.com/publication/frame.php?i=718545&p=&pn=&ver=html5&view=articleBrowser&article_id=4098831
[1]Stanislaw Supplitt, Karpinski, P., Sasiadek, M., & Izabela Laczmanska. (2021). Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine. International Journal of Molecular Sciences, 22(3), 1422–1422. https://doi.org/10.3390/ijms22031422
[2]HTRA1 protein expression summary - The Human Protein Atlas. (2014). Retrieved March 28, 2024, from Proteinatlas.org website: https://www.proteinatlas.org/ENSG00000166033-HTRA1
https://editions.mydigitalpublication.com/publication/frame.php?i=718545&p=&pn=&ver=html5&view=articleBrowser&article_id=4098831
[1]Stanislaw Supplitt, Karpinski, P., Sasiadek, M., & Izabela Laczmanska. (2021). Current Achievements and Applications of Transcriptomics in Personalized Cancer Medicine. International Journal of Molecular Sciences, 22(3), 1422–1422. https://doi.org/10.3390/ijms22031422
[2]HTRA1 protein expression summary - The Human Protein Atlas. (2014). Retrieved March 28, 2024, from Proteinatlas.org website: https://www.proteinatlas.org/ENSG00000166033-HTRA1